How to calculate the concentration of undiluted samples (cells lysate)?

To determine protein concentration of unknown samples (whole cells lysate) coming through the standard curve that has to be plotted before, I know that that can be obtained after multiplying the...

06 December 2020 3,580 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

What makes yellow color appear in phosphorus sample after autoclave digestion?

I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I...

25 January 2021 206 3 View

Disposing Congo Red Agar?

Hi all, Hope you are well. My lab is planning to use some Congo red agar (0.01%) for Shigella flexneri, and we are wondering how do you dispose of the used agar plates. The Shigella contaminated...

13 January 2021 9,823 2 View

How can Sea water impact the hydrodynamic size of Nanoparticles differently than the same Sea water if it is autoclaved?

I am using the same sea water as autoclaved as well as non-autoclaved after adding the nanoparticle. I am getting different Hydrodynamic size for the same nanoparticle.

06 January 2021 3,184 3 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

How to get dry petri dishes in glass after wet sterilization?

Hi, I warping my plates with paper, later put them in the autoclave (use wet sterilization cycle) but still some plates are get wet after ends the cycle. How to get dry the petri dishes? and if...

13 December 2020 8,062 4 View