Abdulmajid Alanazi

3 Questions 3 Answers 0 Followers

Questions related from Abdulmajid Alanazi

The effect of autoclave on glycerol, EGTA and LiCL?

I have three solutions (glycerol, EGTA and LiCL) that were already autoclaved, and I intend to use those in ChIP experiment .. My question to you is does autoclave affect the ingredients of those...

30 May 2022 8,541 1 View

How to calculate the concentration of undiluted samples (cells lysate)?

To determine protein concentration of unknown samples (whole cells lysate) coming through the standard curve that has to be plotted before, I know that that can be obtained after multiplying the...

07 December 2020 3,733 3 View

What is the appropriate volume of protease inhibitor cocktail/ Phospho-Stop to add before cell lysis?

I am going to extract proteins, and for that, I will use a protease inhibitor cocktail and Phospho-Stop (from Sigma). For protease inhibitor stock solution (2x conc.), it is mentioned through the...

27 October 2020 4,483 3 View