What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Can someone recommend me a classic book of liquid crystals?

I am currently into liquid crystals and have no related background. Can someone tell me which book is the most instructive one to learn basics of liquid crystals? I am more interested in different...

22 November 2020 4,362 6 View

How does the influence of metal ions on the fluorescence intensity of a compound?

We found an interesting phenomenon that metal ions could not influence the fluorescence of a compound when the compound was dispersed in solvent. However, the fluorescence of the compound was...

31 October 2020 6,568 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Fluent how to draw a graph of coordinate changes over time?

When simulating waves, it is necessary to detect the coordinate change at a certain point of the wave surface, but there is no in fluent.

04 October 2020 1,541 1 View

How to quickly know the new research area landscape and find a research gap in this new area?

As a Ph.D. candidate who enters a new research area that differs from the research topic in the master period (e.g. shift form crop breeding to soil ecology), how to quickly know the new research...

23 September 2020 8,131 5 View

How to build epoxy model in GAFF for Lammps?

Dear all, I am a beginner in Molecular Dynamics. I build a pre-reaction epoxy model using Materials Studio and plan to perform the cross-linking procedure using Lammps. However, the msi2lmp tool...

26 August 2020 7,697 1 View

How to detect brown pigments generated in red wine aging process ?

During aging or storage, the concentration of monomeric anthocyanins decreases rapidly, while low level of polymeric anthocyanins generates, and the color of wine turns brick-red gradually. In...

17 July 2020 6,032 4 View

How to define the particle agglomeration in composite materials or foundry alloys and measure its size distribution quantitatively?

28 June 2020 7,614 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Magnetismic Gravity in the universe?

MAGNETISMIC GRAVITY What is magnetism? Something magnetic has a + and - charge. If 2 + or - are pushed if + and - are pulled. But what is the main reason for this? Why does magnetism have a pull...

21 February 2021 9,538 3 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

Can the intensity of this broad band in XRD mask other crystaline phases present?

I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....

19 February 2021 8,414 4 View