types of culture media for stem cell which provide suitable environment ?
Dear Hasnaa
different culture media are available for stem cells culturing , if you are to start a new culturing experiment your media of choice would stock available media in egypt ,DMEM is a good commercially available and relatively economic for a startup
DMEM is suitable for research work
there is problem in flow cytometer analysis of platelet microparticles
11 December 2015 1,125 5 View
to investigate prenatal some hereditary diseases , the best method to isolate free fetal DNA
09 October 2015 2,886 1 View
in genetic studying the best results can obtained from which one micro RNA, RNA, DNA
09 October 2015 9,468 5 View
there is some difficulties in study the platelets by flowcytometry , so the best phlorochrom used?
05 June 2015 10,021 0 View
As RNA is unstable so many problem arises in separation of samples so can DNA assayed
05 June 2015 4,387 2 View
I want to study the effect of method of delivery on fetomaternal circulation, the ideal indicator is the fetal RBCs in maternal blood, by flow cytometry I found a problem that the expression is high
02 March 2015 8,127 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View