Which is the ideal method to detect the platelets microparticles contamination of FFP by flow cytometer ?

11 December 2015 1,038 5 View

How to detect free fetal DNA ?

09 October 2015 2,790 1 View

Which is more better in investigate a certain mutation is micro RNA, RNA or DNA ?

09 October 2015 9,365 5 View

The ideal method for QC the flowcytometry?

there is some difficulties in study the platelets by flowcytometry , so the best phlorochrom used?

05 June 2015 9,928 0 View

If detection of genetic mutation is better assayed by DNA extraction or RNA method?

As RNA is unstable so many problem arises in separation of samples so can DNA assayed

05 June 2015 4,298 2 View

How can I detect fetal hemoglobin in maternal blood by flow-cytometry?

02 March 2015 8,025 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View