01 January 1970 1 2K Report

I need The Bar-On model of emotional-social intelligence (ESI) questionnaire(*scale).

Please help me

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The harmful Dye pollution from industrial processes such as textile generated how to remove with magnetic biochar ?

07 July 2024 8,118 1 View

Can I purify mononucleosome from T-cell using a kit followed by SEC superdex 200 10/300 column chromatography for CryoEM ?

I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...

19 May 2024 7,170 1 View

How can I prepare a loading dye that also act as a stopping buffer for an enzymatically degraded DNA, for analysis on a sequencing gel?

I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...

17 April 2024 8,025 6 View

Recently I am struggling to purify the full-Length human PARP1 using both pet28a and pet21b vectors. If anyone is getting good yield, protocol please?

Purification protocol for human PARP1 with good yield.

13 March 2024 9,746 3 View

Why doesn’t the FRAP reagent + sample turn blue?

I have prepared the FRAP reagent according to Benzie & Strain (1996). My samples are essential oil nanoemulsion. However, after adding the FRAP reagent into the sample, it doesn’t turn blue....

05 February 2024 2,765 1 View

Is the breeding in fish is governed by Genes ?, G X E Interaction ? or Environmental factors alone ?

Tilapia and Common carp can breed many times while Indian Major Carp breed mostly during monsoon, then is it due to Genetic factors or environmental reasons?

13 November 2023 9,424 2 View

How to use two different GEO datasets in one transcriptomics analysis ?

I want to use two different datasets where one data will be for control while another data will for test samples. How can I use it. What will be the protocol including normalisation. Kindly help...

11 November 2023 4,388 1 View

What could be the right method of doing siRNA knockdown of my protein of interest ?

Dear all, I have been trying to knockdown using siRNA (duplex) targeted against my protein of interest (# 2 lane shown in the image) and a random non-target siRNA ((# 1 lane shown in the image)...

29 September 2023 765 2 View

Oxygen, nitrogen peaks in GC during pyrolysis?

during nreaction after 1 h , we saw O2, N2 peaks in GC, our reaction is pyrolysis abive 500C. It is hard to figure out where these compounds are coming either from the reactor or the catalyst?

31 August 2023 6,323 3 View

Which software is best to generate begomoviral genome map? What is the best software to generate a begomoviral genome map?

Dear Researcher, Different software tools can produce maps similar to the ones above. How can I find the best software for making genome maps like this?

22 August 2023 1,525 1 View

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