I need to make a 10mM stock solution of Texas Red (Sulforhodamine 101 acid Chloride) - dissolved in 5% DMSO, 95% DI H2O, but have no idea how long it would be stable for if stored at 2.0 - 8.0C.
Can anyone suggest a suitable expiry period?
I really do not understand why have you used a 5%DMSO. The dye is readily water soluble. In fact that is one of the reason why it can not be fixed with routine mordant and it bleeds off easily. Secondly, regarding the stability of the dye in water: it is pretty stable and should be usable for atleast 7 days when stored at 25 to 28C.
Thanks for the quick response Jai.
This is the first time I'll be using Texas red, I'm merely following part of a procedure I found in an article (Comparison of various staining methods for the detection of Cryptosporidium in cell-free culture. Boxell, Hijjawi et al, published 2008).
I have now read that DMSO may alter / damage cell walls. As I intend to use antibody-bound FITC following staining of Cryptosporidium oocysts with Texas red, perhaps it would be better to avoid the use of DMSO... especially given your statement re. solubility.
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