I operate a bioreactor at 50 Celsius, whose heating system failed. Upon fixing the heating system within the next couple of days after failing, I noticed that there is a quite a bit of water that the reactor with drew from water displacement reservoir (to measure volume of biogas produced) the next day. From what I interpret, when the heating system kicked in, the temperature rose from 25 C to 55C, which might have resulted in expansion of the inside the bioreactor and then drew water into the reactor to equilibrate.

Also, I tried removing the excess liquid from bioreactor, and when I did, reactor draws more water as I remove, to maintain its levels. More sort of a level controller based on pressure.

I did fix all the problems now after troubleshooting. I am trying to understand the science/ principle and mechanism behind all this. I'd appreciate your insights on this!

Note: After water entering the reactor, level rose to a point where the tip of the tube connected to water displacement reservoir touches the liquid level.

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