I have found a protein that associates with the outside of bacteria. I am interested in measuring the effects of this binding on the bacterial membrane. Dye influx/efflux experiments using flow cytometry are planned. What other properties or techniques could I use to probe the effect of binding?
It binds gram negative bacteria. I am unsure of the function of the protein when it is bound to the bacteria. I want to measure multiple parameters to determine the effects of binding on the bacteria. We know that this protein is a lectin and binds galactose but are unsure of what the protein does.
Quite an important and interesting characteristic of cellular membranes is the degree of hydrophobicity. It can be measured really simply and quickly using the Microbial Adhesion to Hydrocarbon Test (MATH/BATH). What bacterium is it/Why are you studying it? Membrane hydrophobicity affects adhesion/attachment and biofilm formation, which can be important in pathogenicity or for industrial applications.
Klebsiella pneumoniae and Bacillus subtilis mainly. I want to measure various membrane parameters and see how the binding of this protein changes these. Hopefully this will help determine the function of this protein.
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