Where can I find these two papers?

14 January 2021 1,572 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

Is there any ingredient I an add into formula to slow down gas generation from Vitamin C?

I know Vitamin C is unstable and tends to be oxidized quickly. The problem here is I made a Vitamin C beverage at pH 4-5, and it starts to generate gas in the packaging, especially at high...

13 December 2020 6,055 1 View

Why use STOP solution in ELISA?

At the last step for data analysis, we always use a STOP solution to stop the TMB oxidized by HRP. Why do we have to stop the reaction? Couldn't we measure the color wavelength of...

17 November 2020 5,177 3 View

Why case-controlled study is more bias prone than cohort study?

I noticed from several articles saying that case-controlled study is more bias prone than cohort study (in the context of cancer early detection using molecular marker). I spent sometime to try to...

17 November 2020 1,878 4 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

Is RNALater (Qiagen) or Nucleoprotect RNA (Macherey-Nagel) compatible with High Pure Viral Nucleic Acid Extraction Kit?

I am planning to preserve fresh bat guano in either RNALater or Nucleoprotect RNA but my lab has an excess of High Pure Viral Nucleic Acid Extraction Kits. Any idea if both reagents are compatible...

14 October 2020 2,848 2 View

Hi Dear, Could someone figure the pH out?

There are two Triz-base solutions, the pH of solution #1 is 6.85, the pH of solution #2 is 9.97. What is the pH after mixing the same volume (50 ml) solution together. Please figure it out step...

29 September 2020 1,741 6 View

Is standard deviation of residuals of Markov switching GARCH model regime specific?

I'm using MSGARCH package in R. By return_data/Volatility(fit.model), I get the residuals. When I calculate the standard deviation of the residuals, it turns out that it's close to 1 for all...

14 August 2020 3,132 6 View

How accurate is the shear modulus measured using PROCEQ DPC transducer of 40kHZ frequency on 100mm concrete cube specimen?

It is required to evaluate shear modulus of concrete cube specimen of 100mm size. Since the diameter of DPC transducer is around 84mm, I would like to know whether it can give reliable shear wave...

02 February 2021 1,052 3 View

How do you improve retroviral expression from PG13 cells?

I have been trying to express a gene (GFP) in a retroviral system by transfecting human Phoenix-ECO cells, and transducing murine PG13 cells with the supernatant. I then sort GFP-positive PG13...

18 January 2021 476 1 View

Do I need to transfect FuW-tetO-MCS and FUW-M2rtTA plasmids together in HEK293T or separately?

I want to generate a cell line expressing my gene under an inducible promoter. I chose FuW-tetO-MCS but I also need FUW-M2rtTA for the induction. So do I transfect both plasmids together in...

12 January 2021 8,501 2 View

Best time-point to determine changes in mRNA via qPCR?

I am trying to determine what best time-point to harvest my cells, HMVEC, after transduction with long non-coding RNA to determine changes in mRNA levels, specifically VEGFR2/KDR. I am capable of...

19 December 2020 4,865 2 View

Is there any way to calibrate transducer without needle hydrophone?

Is there any way to calibrate transducer without needle hydrophone? Also any one know low cost hydrophone as precision acoustic is quite costly.

16 December 2020 1,070 2 View

How to convert mV output into MPa?

I am working on acoustic characterization of microbubbles using two transducers arranged at 90 degrees. During calibration, I am receiving response in dBm or mV. How I can know the PNP of my...

14 December 2020 1,814 5 View

Modeling and Simulation of Radial Piezoelectric Transducer?

I need some one working in comsol

10 December 2020 8,442 3 View

Transduction of mouse primary neurons with AAVs, protocol?

Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...

10 December 2020 7,900 2 View

How to reconstruct a Lamb wave by a series of pulses superposed?

Lamb wave is useful for solid structure health monitoring, and it consists many modes. Unlike ultrasonic bulk wave, when given a pulse excitation (for example, 5-cycle sinusoidal pulse), only...

08 December 2020 5,440 8 View

Hi can anyone recommend a synovial cell line?

Hi, I am looking for recommendations for a synovial cell line that is transfectable and transducable. We are currently not interested in working with primary cells. Thank you in advance....

01 December 2020 1,894 3 View