HPLC - Chemstation calibration curve?

Hi everyone, I know I'm asking a lot but I'm learning HPLC for the first time. I'm working with a simple assay containing three simple compounds that separate well. I've recently inject standards...

02 March 2019 3,091 4 View

Toxic protein causing cell lysis?

Hi everyone, I'm currently conducting protein expression trials with a difficult to express enzyme. I'm trying a range of induction times, temperatures and induction OD. I notice that at a lower...

02 March 2019 327 5 View

Preparing PNP-acetate for enzyme assays?

Hey everyone, I'm working with an esterase at the moment and I want to preform a pnp-acfetate assay. I'm just curious as to how people here prepare the stock solution of PNP-acetate. Basically...

10 November 2018 6,307 3 View

Improving sequencing results?

Hi everyone, I'm sequencing a gene post site directed mutagenesis and so far I'm getting horrible results with poor coverage. For the same gene I sequence twice using the T7F and T7R primers. I...

05 June 2018 8,908 3 View

Tips for getting gene mutants sequenced?

Hi everyone, I'm trying some site directed mutagenesis at the moment and having problems with the sequencing. I'm new to sequencing so I thought I'd try to get some tips. I'm currently using...

31 December 2017 700 2 View

Can anyone recommend a good protocol for site directed mutagenesis or should I use a kit?

Hi guys I'm looking to use SDM to change a single residue in an enzyme I'm working with. I'm new to this so I thought I would ask advice. Is there a current protocol that works best or should i...

10 November 2017 625 5 View

Question about stability of PNP substrates in kinetics assays?

Hey guys I'm working with PNP-acetate and PNP-butyrate, two model substrates for esterase/lipase reactions which yield a product which absorbs at 405 nm on hydrolysis. All of the assays I've...

09 October 2017 9,094 5 View

Protein migrating at lower molecular weight, proteolysis or wrong protein?

Hey guys, I've recently cloned a protein into a vector and attempted expression. Colony PCR and gels confirmed an insert of an appropriate size was in the colony that I selected for protein...

05 June 2017 162 5 View

Removing contamination from DNA

Hey guys I've been using the Qiagen gel extraction kit to purify my DNA post restriction digest. I seem get very low yield but my big problem is the purity of the DNA product. When I spec it I get...

05 June 2017 1,461 4 View

Can I use CRISPR to knock out a gene in mycobacterium?

02 March 2017 1,193 25 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View