Hello,
I was wondering if anyone knew in what channel TAMRA is detected in a CFX384 system. It is not in the settings. I have been googling and think it may be detected in channel 3? I would like to be sure about this. Thank you to anyone who knows!
I used BLAST to check for the specificity of my primers (for RT-qPCR) and could only find the desired product of 67 bp. After analysing with SYBR Green I foundnd two different melting curves for...
18 March 2020 5,176 4 View
Hello, I'm doing research about algae oil as a resource for omega-3. I'm writing a method about the oil extraction from algae. I found different ways of doing this (wet and dry, dry by using algae...
06 January 2017 4,189 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...
31 July 2024 6,484 1 View