My PMA/IONO cytokines stimulation is not enough I mean I have 15% of cytokine while before we used to have up to 80% what would be the problem please?

I tried with twice as concentrated PMA/IONO, tried also 4h and 5h incubations, tried different aliquots of PMA/IONO (but same concentration of course) but it doesn't work ! any suggesting please...

11 October 2023 5,736 4 View