How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

Just face a Comsol issue dealing with magnetically hyperelastic material?

Hello all, I am just newly doing a simulation to test the deformation status of a magnetized rubber-like material, which is in-compressible, under a shifting magnetic field. Try to find the...

22 January 2021 7,427 2 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

NADP+/NADPH in bacterial cell lysates?

Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...

20 January 2021 232 2 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

Do anyone have experience with zymo research yeast plasmid miniprep?

Hello, I am new to yeast research. I am trying to rescue plasmid from yeast and have tried several protocols developed by researchers. But the results are not promising. I ended up buying this kit...

06 December 2020 1,251 2 View

Why are all the cells are stained by EdU when performing EdU assay?

I was wondering whether there is anyone else have this problem? As shown in the images, almost all the cells are stained with EdU, some are whole nuclei (circle 1), some are puncta inside (circle...

02 December 2020 6,911 4 View

Would there loss some bases after the repairment to the viscous ends by using T4 DNA ligase?

In constructing shRNA plasmids, I want to shorten the size of the plasmids, so I want to use two restriction enzymes for restriction digestion, and then use T4 DNA ligase for complement and smooth...

25 November 2020 3,366 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View