I don't know, if competent cells and transformation protocols used for E. coli also applied to anaerobic bacteria such as Clostridium acetobutylicum?
I'm no Clostridium expert, but I found a protocol for transforming Clostridium dificile with plasmid DNA by allowing E. coli to transfer the DNA to C. dificile via conjugation.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615975/
Thanks James.
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