How to calculate the IC50 of combination-drug of 2 different compounds (synthetic+ active compound)? How to calculate the Combination Index (CI) of combined drugs?
The IC50 of the drug combination is calculated from this proportion:
Formula 7, page 629
For this, the proportion of each substance in the combination is calculated. For example, 20 and 80%, i.e. 0.2 and 0.8. This fraction is then multiplied by the IC50 of each drug.
Combination Index (CI) - formula 17, 18 (page 632). Dm1 and Dm2 - IC50 for 1 and 2 drugs.