Is there an programm which includes questionnaires, items and picture presenatation?

Dear all, i have to set up a task for facial emotion recognition which combines questionnaires and a picture presentation. Unfortunately i have no programming skills and could not set a program...

10 November 2020 4,516 3 View

Cell structur in poplar - vessels or rays?

Dear All, I am currently trying to understand the different cell types and structures in poplar wood slices, and I have difficulties distinguishing vessel members from rays in the tangential...

12 October 2020 2,276 2 View

Does anyone know what is the best way to transfect HEK cells with many constructs (e.g. 15-20)?

I am trying to transfect HEK cells with 15-20 different plasmids and it seems there is some kind of competition for expression. I am currently using Lipofectamine 2000 and transfect each plasmid...

24 February 2019 6,360 3 View

Best RNA extraction method for full-length cDNA?

I'm trying to generate full-length cDNA of my gene of interest (7kb) but have trouble doing so. I was wondering if maybe there are certain RNA extraction methods that are better suitable for this...

29 January 2019 4,774 4 View

How to prepare a 0.75 M sodium citrate solution with pH 7.0?

I want to prepare a TRIzol-like buffer for RNA extraction. In the buffer recipe it's written that I have to prepare a 0.75 M sodium citrate stock solution with pH 7.0. Initially, I only wanted to...

23 October 2018 9,159 6 View

How to make standard curve by using gDNA and plasmid(viral vector) for lenti-virus copy number?

I want to calculate copy number(lentivirus gene in genes of cells) So, i will use two standard curves using WPRE(vector) and Albumin(gDNA) primer. 1. Should plasmid dna be linearized? 2. If so,...

17 June 2018 7,465 3 View

How can I increase protein yield in protein expression?

Hi guys, I'm currently struggling with protein expression... my POI shows very low yield and it seems like the protein expression induction is not very efficient... I already sequenced my...

03 May 2018 9,449 21 View

Which filter size should I use for sterile-filtration of aqueous glycerol solutions (10%)?

Hi everyone, I heard that glycerol is better sterile-filtered than autoclaved. Can anybody tell me the appropriate filter size? Thanks in advance!

29 April 2018 5,626 3 View

HV Transmission pole (possible scaling and function?)

14 February 2017 7,845 3 View

How many ug of exosomes are needed for EM analysis?

Hi, I am planning to do EM analysis on exosomes derived from neural progenitor cells. I have quantified the amount of exosomes that I got from my prep by BCA assay and it gave me a concentration...

05 February 2017 7,119 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View