Are there any publications where streptavidin's stability against organic solvents is systematically investigated?
Dear Martin,
Streptavidin is a tetrameric protein produced by Streptomyces avidinii . It binds up to four covalent interactions known between proteins and molecules of a small water-soluble vitamin, D-biotin (vitamin H), with a remarkably high affinity. The dissociation constant (K ) of the complex between streptavidin and biotin is estimated at approximately 10-15 M, which is one of the tightest non-interaction known between proteins and their ligands. For example, it can maintain its functional structure at high temperatures, extremes of pH, and in the presence of high concentrations of denaturants and organic solvents. This protein has exceptional stability against proteolysis.
M. Wilchek, E.A. Bayer, Methods Enzymol. 184 (1990) 5.
M. Wilchek, E.A. Bayer, Methods Enzymol. 184 (1990) 14
For more on this topic, please see the review article contained in the following link:
https://structure.bu.edu/sites/default/files/sano1998.pdf
Other links which may be important are:
http://www.ncbi.nlm.nih.gov/pubmed/20939005
https://en.wikipedia.org/wiki/Streptavidin
https://www.thermofisher.com/ps/en/home/brands/product-brand/dynal/streptavidin-coupled-dynabeads/dynabeads-streptavidin.html
Hoping this will be helpful,
Rafik
Thanks for your answer Rafik, but unfortunately it doesn't help. Of course I know that streptavidin is quite stable under organic solvents but I'm more interested in the question: How far you can go? What are the limits in terms of concentrations or vol-%? Which solvents does streptavidin tolerate better?
Hello Martin, I am interested in how your final finding about this question. Do you get a good answer to share? Thanks.
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