What is the best way to isolate proteins which are located in the endosomes and the ER membrane?

For one of my projects, I need to perform WBs on cell lysates. The protein of interest (LRAT) is located in the endosomes and the membrane of the ER. What is the best protocol to isolate proteins...

25 November 2020 969 3 View

How to increase the signal of the protein standard when performing western blot?

Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...

06 October 2020 1,107 5 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Can someone help with human aortic smooth muscle cells (HASMC)?

Hello, I just started working with human aortic smooth muscle cells (HASMC) for the first time and experienced contamination problems. I purchased Lonza's human smooth muscle cell systems with...

17 February 2019 809 2 View

Isolating high molecular weight DNA from a single feather?

I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...

14 August 2018 5,190 2 View

Why an actin band does not appear on gel after PCR for BMM diffrerentiated into osteoclasts?

Hello everyone, I am currently trying to set up at a new lab in order to begin experiments on osteoclasts. Before starting anything, I wanted to make sure that BMMs have indeed differentiated...

17 May 2018 3,308 3 View

Is there any significant difference between Silver and Au as electrodes for polymer thin films dielectric constant measurements?

12 June 2017 3,702 3 View

Does anyone know any questionnaires which measure the use of social media?

I need a questionnaire to measure individuals use of social media e.g. how much they use it etc.

25 October 2016 1,045 3 View

Why do Galleria mellonella fail to pupate sometimes?

I'm using wax moth larvae for some soil baiting experiment, and I'm not sure why some larvae seem to "forget" to moult one last time, and die in failing to pupate, stuck in hardening exoskeleton....

01 May 2016 5,175 2 View

What is the best way to quantify gene expression for 22 genes in 1200 samples?

Hi all, I would like to study the gene expression levels of 22 genes in 1200 different samples. Since normal qPCR is humanly not doable here (26400 reactions) I would like to know if anyone knows...

14 March 2016 5,576 3 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View