Storing cDNA at -80ºC?

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In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another?

Will the leadership style used in the U.S. be successful in Australia, or will the Australians respond better to another? Which leadership training methodology would be most successful with your...

14 July 2024 173 4 View

How can I solve this problem with my western blot?

I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please

02 July 2024 8,715 2 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

How Artificial Intelligence (AI) can change the way to write a thesis?

How Artificial Intelligence can change the way to write academic thesis under master and Pd.D. levels are affecting the originality and knowledge of future academics.

09 June 2024 1,156 9 View

Laser for perovskite module?

Hi, I'd like to know if someone knows about a company where I can buy an affordable laser-scriber for perovskite films and another thin film for the fabrication of modules. Many thanks,

31 May 2024 2,841 0 View

Help! Alizarin Red not staining?

I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...

28 May 2024 4,068 2 View

Has anyone used the MEMS and Nanotechnology Exchage (MNX) foundry service?

I have been trying to contact them for about 2 weeks now and I have received no response. I am wondering if they are still active. If not, could anyone suggest a similar foundry service to do some...

12 May 2024 2,100 0 View

What is my ResearchGate code?

what is my ResearchGate code?

05 May 2024 9,749 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

How can I extract glycosides from mangrove leaf samples without an incubator that can reach temperatures above 60°C?

I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum...

13 July 2024 3,686 0 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Hi all, what is the interpretation of the results of this gel electrophoresis image?

We aim to measure the RNA expression of p53 and K-RAS biomarkers in colon cancer samples. We extracted the RNA from Rneasy FFPE kit and converted the it to cDNA and did real-time PCR using Cyber...

08 July 2024 7,781 6 View

How to properly dilute RNA for cDNA?

Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...

07 July 2024 9,206 4 View