I perform fEPSP recording on hippocampal slices and I have been struggling with a low frequency noise so strong that it affected the slope of my fEPSPs. Finally, I found out that the noise was caused by the stimulator battery and once I've grounded it everything was fine!
Did anybody had a similar experience? Do you usually ground the battery?
Do not ground the battery! This will almost certainly completely disable the isolation provided by the simulator, and in essence you will be applying large voltages to the input stage of your amplifier. This is one of the main reasons we use stimulus isolators!
Instead, follow Mao Yeh's instructions. Try shielding (and grounding) the wires leading from your stimulator to the bath (in fact, I'd use coaxial cable if I could find some). But do not ground the stimulator batteries!
Agree with William M Connelly. If you have a Faraday cage around your setup, put the stimulus isolator inside, it should solve the problem.
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