Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

What are the different subclasses in intermediate seeds?

I am currently working on an endemic tree species, that produces mature seeds (moisture content 51%) which shows both vivipary (30% of total harvested seeds from a tree) and dormancy (~40%) of...

20 January 2021 2,670 3 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Is it machine learning models that have replicability capability?

We are doing the prediction of soil nutrients using spectral information by applying machine learning models. More than 300 soil samples were used from one district for analysis. We got the...

21 December 2020 9,663 3 View

How can I analyse and compare crop yield under different irrigation methods?

I have to compare yield parameters of a tree crop grown under three irrigation methods. But these systems are already established separately in three adjacent fields. Since I can not follow a RCBD...

21 December 2020 3,065 5 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Buckling analysis of a cylindrical shell in Ansys APDL?

In Ansys APDL, We are trying to validate our shell model for nonlinear buckling analysis considering the initial imperfection (as given by Ahmed et. al.) We have attached our results as attached....

13 February 2021 9,200 5 View

How to deal with cross loadings in EFA?

There is an issue of cross-loadings in my output results after running an EFA with varimax rotation. Should I ignore these cross-loadings since these differ by more than .20?

12 February 2021 802 4 View

Is it necessary to include orbital dynamics (translational) in attitude dynamics (rotational) problem?

I want to simulate satellite attitude control with a quaternion attitude representation. thrusters and reaction wheels are the main AOCS controllers. is it Ok to omit orbit dynamics (orbit...

31 January 2021 5,094 2 View

how to add blood and plasma in comsol?

I am trying to model separation of the plasma from the blood on a rotating microfluidic platform. I am not able to add blood and plasma in Comsol. someone please provide me your valuable...

27 January 2021 8,263 1 View

How to rotate an anisotropic stiffness matrix to an orthotropic matrix of cancellous bone?

Hi everyone, recently i have a question about how to calculate the orthotropic elastic matrix of cancellous bone cube. I really need the help!!!! I applied six stress states to the cancellous...

21 January 2021 9,143 2 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

How to choose proper spindle for measurement of thixotropic behavior of ceramic slurry?

Hello, we are studying on thixotropic behavior of ceramic slurry . We will perform the thixotropic behavior analysis of ceramic slurry with the density of 1,7 g/ml by using BROOKFIELD DV2T. How to...

08 January 2021 1,776 5 View

How to rotate crystal structure to match with match another structure of same compound/polymorph?

Say, I have two crystal structures of a particular organic molecule, the crystal structures are basically identical, apart from a rotation and redefinition of the lattice vectors/angles, a simple...

07 January 2021 3,406 5 View

How to formulate the dynamics of the body of a legged robot(quadruped/hexapod) ?

I need to formulate the lagrangian dynamic equations for the hexapod robot's body. The body will constitute 6 DoF, which is 3 linear and 3 angular. I have the coordinates of each corner of the...

24 December 2020 1,222 1 View