For my dissertation I'm validating the method of sonication culture for prosthetic joint Infections and orthopaedic implant infection. Hopefully this method can be used in our lab since this has been repeatedly proved to be more specific than tissue culture. I want to investigate if this sonication method will also be beneficial for detecting osteomyelitis. In the literature there is not much to find on this subject, maybe researchers here can help me with the question if this is possible or maybe there are labs who already use this method?
I recommend to send this question to
Prof. Dr. Andrej Trampuz - PRO-IMPLANT Foundation; Charité Berlin since he is an expert in the field.
Kind regards
SB
Jana van Wijngaarden an Wijngaarden Did you mean native osteomyelitis?
Rodrigo Cuiabano Paes Leme Thanks for your reply! I don't perticulairly mean native osteomyelitis. I mean sonication on bone fragments related to osteomyelitis (bone infection).
Jana van Wijngaarden Welcome! The sonication of bone fragments is not a valid technique. The implants provide a massive amount of biofilm, the best way to survive in a hostile environment. The other manner to evade the immune system is surviving into the osteoblasts, as Staphylococcus aureus does. It reduces osteoblast activity and may cause osteoblast necrosis. Therefore, sonicating bone fragments could not be helpful; this method can not release S. aureus from osteoblasts. Finally, vortexing (a stage of sonication method) cells can lead to mechanical shearing of the DNA. On the other hand, low-intensity pulsed ultrasound (< 5MHz) is helpful for fracture healing - the frequency for implant-related infection diagnosis purposes is 40±2 kHz.
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