What is/are the best solvent(s) for extracting bioactive components of a plant whose phytochemical components have not been explored before? Which protocol would be best for this?
Zafar Iqbal
it depends, on the polarity of the molecules, normally 10%,20% -------50% ethanol is enough for extraction of bio-active constituents. just find, how there is any fragrance, if not then try these solvents ratios, you will get triterpenes, saponins, tannins, etc. keep the temperature low around 60 c then increase slowly up to 100 with each time increase 10C. it is hoped you will get the desired bioactive molecules.
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