Solubilizing insoluble beta Glucan with Zwitterionic detergent ?

01 July 2016 1 3K Report

I have seen some references on Google on this . Does someone have a classic paper reference for this using Chaps or another detergent ? If so can it be done on a large scale 100-200 grams ?

Sanjay Mishra

Yes ofcourse. You may go through the reference:

Mishra, S. and Kamisaka, Y. (2001). Purification and characterization of thiol reagent- sensitive glycerol-3- phosphate acyltransferase from the membrane fraction of an oleaginous fungus. Biochemical Journal 355: 315-322

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