Is anyone aware of software that simulates Phase contrast and DIC microscopy from bright field images. I have read several papers on techniques describing it e.g. Optical Phase Microscopy Allman et al (2002) Microscopy and Analysis that show pictures simulating phase and DIC from bright field microscopy but I can't find the software online, or a commercial seller. Dr Keith Nugent from Australia has published papers on Quantitative phase... and mentions IATIA Ltd Qpm www.iatia.com.au, but the web site is no longer available and I received no reply from Dr. Nugent. If anyone can help in identifying software that mimics DIC and Phase contrast that is available I would be greatful - please include a link to the source if possible.
Dear Professor Berdan, could you kindly let me know if your search was successful ? Maybe you have found a MathLab code or ImageJ plugin ? I am interested in quantifying OPD or dry mass of cells simultaneously with immunofluoresce registration. However, I have not found method other than commercial hardware solutions like Phasics Sid4Bio camera.
Hi so far my questions have not been answered in research gate. I have read several papers showing pictures that it is possible, but authors have not replied to my emails. I own microscopes with DIC and several versions of phase contrast, but the ability to apply these techniques to bright field microscopy images would be great for those that could not afford these techniques - especially DIC contrast which can cost more than $10,000. Image J does not have this feature to my knowledge. I have found that I can simulate blind Deconvolution software using Adobe Photoshop.
https://www.canadiannaturephotographer.com/epifluorescencemicroscopy.html I am attaching one report I read that shows Phase and DIC simulation from July 2002 Microscopy and Analysis - though it is not a refereed paper the images look convincing.
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