Hello everyone, I am planning to do a 13C stable isotope labelling experiment in algae, it is going to be a pulse-chase experiment, where I will be feeding the algae with 13C isotope labelled substrate for 4 days, I will be keeping the cells at a metabolic steady-state by maintaining the cells at a constant optical density (like a chemostat), after maximum isotope labelling, I will be transferring the cells to an isotope free media and deprive the cells of nitrogen to track the fattyacid reshuffling into Triacylglycerol molecules.
What software packages do you people suggest for analysing my LC-MS and GC-MS data?
Maybe the following links can help you
https://www.researchgate.net/post/Which-is-the-easiest-software-for-GC-MS-data-analysis
https://www.researchgate.net/post/Is_there_any_data_analysis_software_that_does_quantification_of_LC_MS-MS_data_exported_in_xml_xls_format
Article Navigating freely-available software tools for metabolomics analysis
https://www.researchgate.net/post/Aligning_GCMS_data_with_retention_times_on_R
A package in R called BiocManager is also used by some for the same
https://bioconductor.org/packages/release/bioc/html/xcms.html
Mr. Potlapalli,
In order to, obtain exact quantitative information about your analyte by mass spectrometry you need (i) instrumental setup software; (ii) free of charge AMDIS software [http://www.amdis.net/]; (iii) calculator; and (iv) Microsoft Excel, which is installed routinely on each computer, respectively.
Why?
Because of, accurate, presice, selective, sensitive and exact quantification by mass spectrometry, currently, can be carried out only within the framework of our own-authored (to me and my co-author's according to the shown authorship below) most recently developed stochastic dynamic theory and model formula, which show linear correlation coefficient between theory and experiment /r/ = 0.99997 - 1 at concentration levels of analytes pg.(mL)-1.
Please, consider works [1,2], in addition to, reference [3].
1] Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller Analytical Chemistry Letters, 10 (2020) 703-721; Received 13 Oct 2020, Accepted 16 Dec 2020, Published online: 28 Jan 2021 Download citation https://doi.org/10.1080/22297928.2020.1865834
[2] Steroids, 164 (2020) 108750 Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller.
[3]Conference Paper Mass spectrometric and quantum chemical treatments of molecu...
(The presentation is available on the Conference's web-page [https://ecmc2021.sciforum.net/] (section: General,) as well)
AMDIS software provides absolute intesity value per span of scan time according to the theoretical framework of our model equation. These intensity values are calculated according to the model equation (references [1-3]) by calculator. The linear correlation between the parameters of our formula and the analyte concentration in solution is carried out by Excel.
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