How do I evaluate data I got from Image Lab Normalization?

Hi, I'm the first one in my lab to use the ImageLab Normalization-tool and so far I'm not sure what to do with the data I received. I tried to standarize one blot with one protein band with a...

03 April 2016 5,179 3 View

Does anyone know what is the control used in ELISA assay? Should coloured product appear in the wells with control?

I am trying to develop my own sandwich ELISA assay for detection of NGAL (biomarker of acute kidney injury). First I immobilize primary antibody (anti-NGAL monoclonal antibody) during the night in...

24 January 2014 3,616 14 View

Social media and identity

has anybody looked at writers/writing & identity in social media? I'm specifically looking at refugee writers who prefer using social media as their gateway.

01 January 1970 8,502 2 View

Best and worst User Interface Design Challenge?

Please present example good or bad User Interface design. Obviously, good UI can make us feel at home and everything out there is just in the right place; while bad UI will make you feel like...

01 January 1970 6,663 9 View

Do you need to have an engineering degree to be a UX Engineer?

User experience roles are considered to be some of the best jobs in the IT Industry. With user experience design becoming more popular and in demand, the need to diversify skills is clear....

01 January 1970 5,553 10 View

Do you agree that Gamification in education can improve motivation and engagement of students?

Everyone loves games. Albert Einstein himself indicated they are the most elevated form of investigation. He knew games are avenues for something deeper and more meaningful than a childish waste...

01 January 1970 3,746 67 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

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22 February 2021 9,932 11 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View