SLAMF7 in T-cell - FAQS.TIPS

01 January 1970 1 7K Report

Has anyone assessed SLAMF7 (CD319) expression on T-cell ex vivo from human samples ? just wondering if a resting period after thawing cryopreserved cells can increase its expression and if yes, how long the rest should be ...

Thanks all,

Michael Förster

Dear Jean Benoit Tanis

Please check the IMMGEN database for expression data on purified T cell populations from PBMCs. Unfortunately, it seems that there is very little SLAMF7 expression on T cells in general, at least for non-activated/ naive cells.

http://immunecellatlas.net/ICA_Skyline.php?gene=TP53&celltype=all&organ=all&datatype=rnaseq&scale=Local

It seems only effector memory T cells show robust expression, but these you might have to re-stimulate, if you can't detect the ca. 20-30% in PBMCs in your healthy donors, as per:

Article Phenotypic and Functional Separation of Memory and Effector ...

Maybe that helps.

All the best & kind regards,

Michael

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