Hi,
I am new to the field of in vitro actin polymerization and was wondering if there was a way to get rid of polymerization nuclei in my purified actin without the use of gel-filtration on a HPLC? Does anyone have any recommendations?
I have received the following output based on my research, which looks at a parallel mediation model with 2 mediators. There is a significant total indirect effect, but both the individual...
17 December 2023 3,658 2 View
If you have an area of several 25's of acres (several 100,000 m²) and your only source of GNSS Information is that of the drone, can there be distortion of the 3D Model / orthomosaic that are so...
28 July 2023 5,345 4 View
Hei everyone, I ll start here a discussion for which I can't find any answer online. We are facing an issue with the water that we use in PCR and qPCR. The problem is that when we use 16S...
27 June 2023 747 7 View
Is there a special reason, why the term "superficial cervical ansa" (Ansa cervicalis superficialis) was removed from official anatomical terminology?
04 June 2023 8,367 4 View
I want to automate the measuring of neurite outgrowth in PC12 cells. Is there any programm or fiji plugin that can measure cellbody length ,neurite length and number of neurites in a unstained cells ?
01 June 2023 7,145 3 View
I have RNA-Seq samples from an experiment in which I infected epithelial cells with bacteria. The experiment was repeated identically 3 times and then once more with slightly altered parameters...
09 May 2023 965 1 View
Hi everyone, I have a question regarding Geltrex vs. Matrigel in the differentiation of iPS cells to cardiomyocyte-like cells. So far we have always used Matrigel and obtained high yields of...
10 February 2022 7,997 0 View
Dear community, When drawing a structural model, I am highly dissatisfied with the final significance values. I obtain only one significant predictor for my dependent variable. If I remove the...
17 November 2021 2,813 7 View
I am searching for gas radiation coefficients, which I intend to take into account with the optical thin model for the calculation of temperatures and exhaust gases from a combustion process....
11 April 2021 9,278 1 View
-Required are DEM/DTM satellite data from mountain ranges without glaciers. For example SRTM 30m. -The data has 3 parameters only. X, Y and Elevation. There is no need for georeference or...
22 January 2021 4,447 6 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View