i try lot more time but not separated my lost two peak ,please give any suggestion
Your baseline noise is very high relative to your strong signal level. What type of detection are you using? Are you continuously degassing your mobile phase?
Please provide some of the details of the HPLC method used (Column type and dimensions, flow rate, mobile phase type & composition (gradient or isocratic?) and time table, injection concentration and volume, detection settings (detailed), temperature and so on) plus injection solvent used.
yes,i am using c18 column ,and mobile phase is acetonitrile and water ,acetic acid-ratio(49;49;2)-temperature- 40 Co,
flow rateis 1ml per min
detector -fluorescence detector ( i use gradient and isocratic)
10ppb-100ppm concentration i try this
20 ul only i injected and i am using shimazu 20 model
Column dimensions please.
If you are running isocratically. switch to a gradient to improve your separation.
i am using gradient also not got and column dimensions is 150*4.6 mm
- Badges
- Science topic
- Similar topics
- Physics
- Condensed Matter Physics
- Solid State Physics
- PEAKS