Without using an ultrasonic cell disruptor, it can gently and massively lyse Escherichia coli. Through a single cycle freeze-thaw method, three types of buffer solutions were used during the operation, each containing components such as β - Mecaptoethanol and glycerol. In addition, Lysozyme and DNase I were also used. The final bacterial lysate was used for nickel ion affinity chromatography purification of recombinant proteins with His tags. Which friend can provide relevant literature materials? Thank you very much.