Why is my bacteria not growing after a transformation?

Hello, I did a transformation a few weeks ago and while only a few bacteria grew, I was able to pick single colonies for a liquid culture. However, I had to repeat this experiment because...

28 July 2020 278 6 View

In public healthcare, how many cases should a psychotherapist to treat per day as a maximum in order to protect healthcare quality?

Do you know any paper, or official report, from any country, stating about how many patients or families should a clinical psychologist or psychotherapist be allowed to treat per day as a maximum...

22 December 2019 2,980 10 View

Why is my tissue detaching after H&E staining?

Hello! I've been having trouble with staining lung and pancreatic tissue. While doing H&E, tissue partially (or completely) detaches. Sometimes, the tissue folds on itself as well. Not sure why...

31 October 2019 8,432 4 View

Is there a resource available that provides Paris Law constants for different types of metals/steels?

I am trying to determine the number of cycles to failure for a solid piece of metal with a 1 inch crack in it. The metal is A36 steel. Case 1: the crack is in the middle of the width on...

04 May 2019 8,332 3 View

Does anybody know how to manually dice a Fused Silica wafer?

We've been trying to manually cut Fused Silica (diameter of 100 mm, thickness of 500um) the way we normally proceed with Silicon wafers: by marking the cut with a diamond tip, cleaving the wafer...

07 August 2018 9,251 1 View

Any advice on a 3D Transmembrane proteins structure predictor?

Hello, Im looking for a software (free if possible) or tool which allow me to predict a 3D structure of a transmembrane protein. The thing is I know where is the TM domain, but I would like to...

11 December 2015 3,359 5 View

Are high-mountain medicinal plants popularly considered more effective than lowland ones?

At least in some Spanish territories people used to consider high mountain plants to be more effective than lowlands ones (regarding medicinal plants), even being the same species. I would like to...

22 October 2012 769 24 View

In best practices, what is the correct way to formulate an optimization problem

An optimization problem must be expressed mathematically with the least number of variables or the mathematical formulation that allows to demonstrate its optimal solution, however it requires to...

01 January 1970 1,115 7 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Why is ethics significant in business and how should one control unethical behavior ?

1. The significance of ethics to business and how to control bad behavior.

21 February 2021 882 14 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View