HPLC - Chemstation calibration curve?

Hi everyone, I know I'm asking a lot but I'm learning HPLC for the first time. I'm working with a simple assay containing three simple compounds that separate well. I've recently inject standards...

02 March 2019 3,091 4 View

Toxic protein causing cell lysis?

Hi everyone, I'm currently conducting protein expression trials with a difficult to express enzyme. I'm trying a range of induction times, temperatures and induction OD. I notice that at a lower...

02 March 2019 327 5 View

Preparing PNP-acetate for enzyme assays?

Hey everyone, I'm working with an esterase at the moment and I want to preform a pnp-acfetate assay. I'm just curious as to how people here prepare the stock solution of PNP-acetate. Basically...

10 November 2018 6,307 3 View

Improving sequencing results?

Hi everyone, I'm sequencing a gene post site directed mutagenesis and so far I'm getting horrible results with poor coverage. For the same gene I sequence twice using the T7F and T7R primers. I...

05 June 2018 8,908 3 View

Tips for getting gene mutants sequenced?

Hi everyone, I'm trying some site directed mutagenesis at the moment and having problems with the sequencing. I'm new to sequencing so I thought I'd try to get some tips. I'm currently using...

31 December 2017 700 2 View

Can anyone recommend a good protocol for site directed mutagenesis or should I use a kit?

Hi guys I'm looking to use SDM to change a single residue in an enzyme I'm working with. I'm new to this so I thought I would ask advice. Is there a current protocol that works best or should i...

10 November 2017 625 5 View

Question about stability of PNP substrates in kinetics assays?

Hey guys I'm working with PNP-acetate and PNP-butyrate, two model substrates for esterase/lipase reactions which yield a product which absorbs at 405 nm on hydrolysis. All of the assays I've...

09 October 2017 9,094 5 View

Protein migrating at lower molecular weight, proteolysis or wrong protein?

Hey guys, I've recently cloned a protein into a vector and attempted expression. Colony PCR and gels confirmed an insert of an appropriate size was in the colony that I selected for protein...

05 June 2017 162 5 View

Removing contamination from DNA

Hey guys I've been using the Qiagen gel extraction kit to purify my DNA post restriction digest. I seem get very low yield but my big problem is the purity of the DNA product. When I spec it I get...

05 June 2017 1,461 4 View

Can I use CRISPR to knock out a gene in mycobacterium?

02 March 2017 1,193 25 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View