I did the hemolytic test, The result showing after 24 hours indicated that the bacteria have gamma-hemolytic (non-hemolytic), but I kept the plate in the incubator for 48 hours, then the clear halo zone showed around the colony (Beta-hemolytic). So which result should I use? I read some papers and normally they did incubation for around 16 -24 hours.
Another found was that 75-90% of my isolates from the fish gut have Alpha or Beta hemolytic. and almost 100% of my isolates with antibacterial activity are hemolytic strains. So I would like to ask two more questions:
1. Is hemolytic really a necessary safety test for probiotic due to many bacteria with hemolytic is available in the environment and we still can scope with them?
2. hemolytic activity and antibacterial activity are in correlated or not?
Thank you very much for your suggestion and comments!
With regard to the second paragraph, I think there is correlation between hemolytic and antibacterial activity
Sumod Kadhem Hi, Thank you for your comment. But do you have any evidence for that? Thank you!
If Your bacteria is showing zone on the plates in between the incubation of 24 hours then there is no need to provide the more incubation.
Thank you
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