Calculate pH after addition of base?

04 February 2021 2,232 4 View

Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

How does one create IFORM contours?

Hello: Can anyone assist with plotting IFORM contours. I have been trying to plot contours using Hs and Tp as variables. The marginal distribution is a GPD-Kernel density and the conditional...

12 December 2020 4,355 3 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

What are your tips to write the discussion section of your scientific work in an efficient way?

Writing the discussion section is always the scariest part of a paper or a thesis. I came up with some ideas to make it less difficult, but would like to hear other tips! Here are my two bits:...

04 November 2020 6,808 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

How can I determine the total antibodies in the serum of bovines with ELISA?

I want to determine if there is a difference in the amount of total antibodies in the serum of different bovines, I was thinking of doing it with Elisa sandwich. Has anyone tried it? What initial...

13 August 2020 2,412 4 View

Dose-response on non-Michaelian response enzymes, how?

Hello For typical dose-response assays, our lab usually uses steady state intervals for defining the difference between control and tested compound. For those assays, we typically use the angular...

23 July 2020 8,662 2 View

What measures have your labs/ institutions put in place to mitigate the impact of COVID-19 on early career-researchers?

Early career-researchers are disproportionately affected by the COVID-19 pandemic in academics, as they often struggle with precarious working situations and they are seeing many doors shutting...

27 May 2020 1,440 1 View

What is the best way to graph an SVM classification in R or Python?

I'm trying to find a way to graph a classification made by the support vector machine algorithm. Something like a PCA or LDA chart would be excellent. Any help will be welcome.

26 April 2020 4,588 1 View

What are current and emerging approaches to visitor monitoring in the context of leisure tourism (rail trails)?

A team of researchers at Southern Cross University in a study on the long-term benefits of developing rail trails. We are currently conducting a literature review of current and emerging...

01 January 2020 891 10 View

What are current and emerging approaches to visitor monitoring in the context of leisure tourism (rail trails)?

A team of researchers at Southern Cross University in a study on the long-term benefits of developing rail trails. We are currently conducting a literature review of current and emerging...

31 December 2019 1,415 5 View

I am a PhD student, i would like to know how can i apply emergy method on ecotourism for example national park?

I am a Ph.D. student, I would like to know how can I apply emergy method on ecotourism, for example, national park?

10 December 2019 8,485 1 View

What's the methods to identify an approach that's been used or applied in an area?

I'm currently working on a research about ecotourism development, and I need to identify the methods that's been used on a related area. Is there any particular methods to do this kind of...

16 January 2019 2,835 2 View

What's the methods to identify an approach that's been used or applied in an area?

I'm currently working on a research about ecotourism development, and I need to identify the methods that's been used on a related area. Is there any particular methods to do this kind of...

31 December 2018 2,468 3 View

How do ecotourism destinations or attractions operationalize carrying capacity? Any interesting case studies in Asia?

Information will be used to develop a mechanism to operationalize site-specific carrying capacity standards

22 December 2018 1,639 1 View

Can you help me?

11 December 2018 6,845 5 View

How do ecotourism destinations or attractions operationalize carrying capacity? Any interesting case studies in Asia?

Information will be used to develop a mechanism to operationalize site-specific carrying capacity standards

11 December 2018 2,220 1 View

Why is Ecotourism important and how we can improve it?

Ecotourism is a tool that provides opportunities - a financially viable tool that provides sustainable solutions to economic challenges and conservation needs of local people; an alternative means...

05 June 2018 6,228 5 View

Why should I become involved in ecotourism?

Ecotourism is a tool that provides opportunities - a financially viable tool that provides sustainable solutions to economic challenges and conservation needs of local people; an alternative means...

09 October 2017 3,381 2 View