I have attached folic acid to my compound by EDC/NHS chemistry. How can I separate folic acid attached material from EDC/NHS solution?
Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...
02 February 2021 2,613 7 View
I have a small dataset. Which machine learning algorithm is suitable for this?
07 January 2021 7,833 8 View
i have found the area of segmented image in pixels. How can i convert into inches
01 January 2021 3,336 7 View
We have segmented a part from grey scale image using image segmenter (app in mat lab ) and stored as matlab structure. from that structure , how can we calculate the area of the segmented part...
24 December 2020 4,040 3 View
What is the current status on regulatory approvals of vaccines meant for COVID-19 according to USFDA, EMA, TGA, etc?
10 June 2020 7,582 5 View
I have mortality chart table but i dont know how to run data in probit software.
09 May 2018 8,693 2 View
My otherwise amorphous material is showing strong XRD peaks when in its nanoparticulate form...cant really guess the reason...need help
02 January 2018 3,668 6 View
Hi all, I noticed small white precipitates formed during buffer exchange using Vivaspin. I am new to protein biology. I am not sure if the white stuff that I see are protein precipitates or...
29 August 2016 4,279 9 View
Hi, I am not very good at computational biology. I am working on a transcriptional repressor protein which is believed to be associated with virulence. I did DNAse 1 footprinting to determine the...
30 July 2016 5,886 3 View
21 September 2015 7,215 3 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
20 February 2021 5,820 4 View
Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...
18 February 2021 371 4 View