I am grateful to friends across the globe for patronage to Semen Fructose Estimation Kit developed by me and manufactured by my family enterprise Maa Vaishno diagnostics. Perhaps marketing and procurement problems have been a bottleneck in use of this method of immense importance in cattle and human infertility.

On National Republic Day of year 2020, I am pleased to declare my Reagent Formulation open to public use.

Diagnostic laboratories may prepare and use my Reagent.

Diagnostic companies may manufacture and distribute; no royalty expected.

Method described below and also viewable at http://indianmedical.net/fructose

Reagents 1. DoctorKC' Fructose Reagent Indole 3-acetic acid LR  0.5 g Thiourea LR 0.1 g Isopropyl alcohol 250 ml Shake frequently and keep overnight. Filter The reagent is infinitely stable at room temperature.

2. Hydrochloric acid LR 3. Standard : 200 mg/dl 5 ml

Procedure

For Semen Fructose Drop Test in evaluation of Sperm Activity, Standard reading is not requird.

1. Pour over 0.005 ml sample, 5 ml Hydrochloric acid. 2. Add 0.1 ml Fructose Reagent. Mix by inversion. 3. Incubate at 50ºC for exact 50 minutes. 4. Cool and read absorbance of Sample and Standard against Blank adjusted to zero with bluish green filter (500-530 nm).

For standard, take 0.005 ml standard in place of sample in step 1. For blank, omit sample/standard addition. Proceed further with both tubes as with the test.

For Semen Fructose Drop Test, use Sample reading of 30 minutes old sample (Abs30) and 240 minutes old sample (Abs240). 

Calculation                       Abs Sample

Fructose = ------------------------------- x 200 mg/dl                       Abs Standard

Fructose Drop using Standard

Fructose at 30 minutes - Fructose at 240 minutes

Fr Drop  = ------------------------------------------------------------------------------------ x 100 percent         Fructose at 30 minutes

Fructose Drop without Standard

                             Abs30 - Abs240 Fructose drop  = --------------------------------------- x 100 percent                                      Abs30