Dear Colleagues
I am doing 16S rRNA sequencing for V4 region. I am planning to run 20 samples on MiSeq for paired end 2 × 150 bp Output at nominal ~ 20,000 depth.
Which MiSeq kit should I use.
I think V2.... But which one of V2? 15 M, 4 M or 1M?
Hi all How can we differentiate in the laboratory an FMD positive result using ELISA or PCR. FMD antibodies positive samples may be because of vaccination or past infection. Also How please...
08 September 2018 3,802 2 View
How we can detect this peptide in Zebra Fish myocardium homogenate? I read that 1 assay is available from Roche called Elecsys proBNP. But I am not sure if we can use it on zebra fish annd also...
01 February 2018 5,200 0 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?
01 March 2021 8,212 3 View
I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...
26 February 2021 9,580 4 View
25 February 2021 3,126 1 View