Scioto Sandstone and Silurian Dolomite, standards for the O & G ?

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Are primary & secondary antibodies needed for flow cytometry to detect EGFP transfected cells?

Hello, I am new to designing flow cytometry experiments. I have some cells transfected with EGFP using mRNA and want to quantify its percentage expressed. I also wish to differentiate between...

17 May 2024 8,527 2 View

Where to find cloud cover estimation during the night for Southern Africa?

Hello RG community, I am looking for remote sensing data on cloud cover estimation on a daily basis but during nighttime over Namibia /Southeast Africa. The ideal spatial resolution would be 1km...

31 March 2024 298 2 View

Does anyone try renaturating inclusion body with copper sulfate?

I am currently doing isolation of protein from inclusion bodies. I solubilize the protein with 8M guanidine hydrochloride (Simpson, CSHL, 2010). I this step I saw no pellet anymore in my sample....

17 March 2024 8,573 0 View

How to form the immune complex using human IgG?

16 November 2023 3,811 1 View

I want to study the male reproductive system for two different seasons. Please guide me how much should be my simple size be?

I want to study the male reproductive system for two different seasons to obtain histology, histometery, morphology and morphometery. Please guide me how much should be my simple size. What would...

22 May 2023 7,177 5 View

Pretest-Posttest Nonequivalent Groups Design: ANCOVA or Change-Score Analysis or Something Else?

I am working on my dissertation, and I am stuck on the type test I should run on my data. Research Design-I have a control and a treatment group where a vocabulary intervention was given to the...

02 April 2023 1,263 4 View

Help with shRNA gene silencing?

I am trying to do a shRNA gene silencing for an esophageal cell line. Does anyone have any specific protocol to suggest?

27 March 2023 6,675 3 View

Can you modify BSA with NHS-PEG4-azide?

I performed a reaction to modify BSA through esterification, but it didn't seem to work. Most of the papers that I've found that attach azide to BSA seem to use maleimide. Is there a reason why...

09 March 2023 4,971 2 View

Policies adopted by state sector to meet the objectives as welfare of citizens?

policies initiatives during past few years

08 March 2023 5,540 2 View

Interpretation of Data ?

How do we as scientists interpret data to provide the most explicit explanation for our research? I am aware that we can present the limitations of our study which can defer our...

15 February 2023 2,867 1 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to Differentiate Tuffaceous Matrix from Clay Matrix in Sandstone Thin Sections?

I am conducting a study on sandstone samples and need to accurately differentiate between tuffaceous matrix and clay matrix when examining thin sections under a polarized light microscope. While...

20 June 2024 3,270 0 View

How to separate sand from marble and graphite?

I'm doing a XRD/XRF analysis for sandstone sample from a borehole, the sample is mixed with other materials from the drilling fluid like graphite and marble, all these material falling in range...

19 April 2024 8,868 1 View

Does water saturation increase P and S wave velocity of rocks? Why?

I performed P and S wave velocity tests for cylinderical limestone and dolomite core samples for dry, staturated (for natural and acid solution treated samples). I expected the P and S wave...

04 March 2024 6,872 2 View

Is it possible(Okay) for iron-oxides to present around 47% in a sandstone?

i have some XRD data in which some samples shows around 47% iron Oxides content in the sandstone. which is confusing for me.

26 February 2024 3,845 1 View

How do we correct measured oxygen isotope values from dolomite?

I need to correct all oxygen-stable isotope values that were measured from bulk dolomite. These values were used along with carbon-stable isotope values to determine the P-Tr boundary.

18 January 2024 4,279 6 View

What is the stiffness/elasticity modulus of highly weathered sandstones?

I'm searching for a range or list of values for the stiffness (in MPa or GPa) for highly weathered sandstones. It would be very beneficial if you could give some information on their petrography.

22 November 2023 4,477 3 View

Does dolomitization occurs through dissolution-reprecipitation at low temperature?

Although geochemical evidence suggests that dolomitization is most possibly through dissolution-reprecipitation at low temperatures and solid-diffusion is suggested to be too slow to be the way...

16 November 2023 4,610 1 View

Best method Sonic log vs Density log to find porosity ?

Between Sonic log and density log method to find porosity of rock formation, which method should be considered ? what are the condition need to be considered to choose between sonic log and...

11 September 2023 7,866 1 View

What methods can be used to analyze monovalent cations on surface not on solution?

In analyzing monovalent cations like Li and Mg on surface (dolomite samples) for precipitates, what methods can be used aside XRD, XRF, SEM-EDS, although the last doesn’t analyze light elements...

08 September 2023 5,623 2 View