Western blot protein of interest falling at a lower weight than expected?

My protein of interest is DAGLa (115 kDa). We received the antibody for this from another researcher who reliably shows bands at this weight. We do see a light banding at this weight, but the...

11 February 2021 6,009 3 View

The use of n-of-1 studies for rehabilitation / therapies (i.e. non-drug/medicine studies)?

Hi there, I am eager to hear from researchers or clinicians who have used n-of-1 studies for the above purposes. They seem to offer advantages over many other study designs, particularly for when...

26 October 2020 328 5 View

PLS-SEM or CB-SEM after qualitative work?

Hello, My research team completed extensive interviews/triads with participants on various topics. The quantitative survey is the next step in our process. From that survey we want to prioritize...

13 August 2020 828 5 View

Are there examples of non-native insects that respond negatively, or neutrally, to anthropogenic disturbance?

I've combed the literature, but am having a hard time finding any examples. Specifically, I am looking for any examples of non-native insects that respond negatively, or neutrally, to...

30 July 2020 8,920 6 View

Nanotechnology question. Getting rid of solvent after LNP formulation?

I'm dealing with really micro volumes and I'm trying to figure out if there are other efficient methods aside from dialysis to get rid of residual solvent for LNP purification. And if not, are...

25 May 2020 6,377 1 View

Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

Do anyone have the experience of testing creatinine (kidney function) by using HPLC?

In my experiment, I want to use serum (mouse) to test the creatinine level via HPLC. 1. During the preparation, I centrifuged the whole blood, and then transfer the supernatant (which is serum)...

17 February 2020 9,679 2 View

If two drugs activate the same apoptotic pathways, can they have an antagonistic effect?

Hello! I'm testing the photochemotherapeutic potential of a drug in vivo. It turned out that treatment with the prodrug caused an effect that was similar to the effect obtained after...

10 February 2020 3,001 3 View

How do you conduct a factor analysis with Multiple Imputation Output in SPSS?

I am working on my doctoral dissertation and have hit a road block with my factor analysis. I used multiple imputation for missing data (n=198 with 5% missing data), but now I am unable to figure...

05 February 2020 6,437 3 View

Why does my analysis keep excluding one of the classes?

We are analysing data on a cluster randomised control trial. We have treated the clusters (classes, N = 8) as fixed effects, thus dummy coding to create 7 different variables. This is because we...

04 February 2020 9,301 1 View

Are there any structural differences between sucrosic dolomite and saddle dolomite?

Sucrosic and saddle dolomite are known to be different petrographically. However, are they different in structure, e.g., under XRD or TEM?

20 September 2020 2,495 2 View

What is the difference between dolomite and ankerite on the basis of PXRD?

I usually face the problem to distinguish between the dolomite and ankerite during the evaluation on powder XRD. Both of these minerals are found within the carbonate sedimentary rocks and belong...

09 April 2020 9,173 4 View

What are the ways to clean the core sample flooded with nano particles?

Core cleaning is a challenging task. In the case of chemical flooding, core samples are generally cleaned by methanol and toluene but in the case of nanoparticles, I am not able to get any solvent...

04 March 2020 5,713 7 View

How can I age sandstone cores?

Hi all, I would like to conduct few experiments on sandstone cores, and I need advice about ageing and which model oil should I use? Kind regards Farida

05 June 2019 1,874 10 View

What is the age of Gulcheru Quartzites & Vempalli Dolomites of lower Cuddapahs?

Recent studies revealed that age of lower Coddapahs is Palaeoproterozoic. Howerver, in majority of previous publications, it is referred to as Mesoproterozoic. In some publications...

03 April 2019 3,468 1 View

How to transfer micro-CT image of sandstone to COMSOL?

I need to convert mesh structure of sandstone obtained from micro-CT raw data to solid geometry, I am trying to do it in COMSOL, but could not do it due to some errors in vertices faces and in...

03 April 2019 345 3 View

Effect of rock abrasivity (los Angeles abrasion test) on wear rate of the drill bit during rock drilling?

As I have conducted los Angeles abrasion test in the laboratory. Three type s of sandstone of varying UCS of Medium grained SS 13 MPa, fune grained SS 17.5 MPa and fine grained SS (pink) 51 MPa....

02 March 2019 7,298 5 View

Can trace element ratios be used to infer paleoredox conditions in sandstones ?

02 March 2019 4,067 5 View

How to determine the mineralogy of sandstone by XRD ?

How to determine the mineralogy of sandstone by XRD ? such as quartz wt% ,kaolinite wt% etc. by XRD data...

11 December 2018 7,044 0 View

Which nano particles could efficiently help with changing the wettability of an oil-wet surface toward more water wetness?

Changing the wettability of an oil-wet sandstone reservoir rock toward more water wetness. Does anybody know something about it? I appreciate your comments

11 December 2018 9,391 2 View