Hi everyone,
I'm doing Saos-2 cell culture study. There has contamination about 2 months and I could not find the cause. There is always a blur in the flask. I share the microscope image after incubation.
Environmental conditions ; 5% CO2, 98% humidity, 37 °C , DMEM-High glucose and F12
Waiting for your ideas ..
Hi
I'd recommend you to start with a fresh stock. Even if u add antibiotics and antimycotic agents in higher concentration than normal, this contamination would not go away. Discard these cells as i can see that cells have not yet adapted to the original epithelial morphology typical of Saos-2 cells indicating that they are in stress and like u said there is a turbidity in the medium so no way that it indicates the presence of debris which sometimes is released in the medium by the cells. So these cells shouldn't be propagated or passaged further or be used for experiment.
For further confirmation use mycoplasma testing kit or DAPI staining for mycoplasma.
For bacteria u can take a few microliters of the media and spread on nutrient agar media or any bacterial broth and observe for enhancement of growth. This should erase any confusions and again start with a fresh stock and keep a watch on your aseptic technique as well.
Hope it helps
şerefe !!