What is the best protocol for preparing an extract from plant parts for GC-MS analysis? Shall we use mid-range polar solvents and/or solvents in the lowest polarity to the highest polarity range? Is aqueous extract suitable for GC-MS?
Dear Dey
Thank you for your question.
Actually plant phytochemical analysis depend on your target..... that means what you want?
I agree with Fornari answer....
Additionally
1. GC-MS maninly used for volatile organic compound analysis.
2. You may use different technique to extract volatile organic compound, for example SDE, HS-SPME, etc
3. I think aqueous extract is not suitable for GC-MS analysis.
Please think your goal and share with us
Interesting question. Having a prior knowledge of the group of phytochemicals you are dealing with will save you time or so. I do not particularly recommend GC-MS analysis of aqueous extracts; this technique works best for volatile solvents which water is not one. Nonetheless, I have seen in some cases where the aqueous extracts are mixed with other volatile solvents and analyzed. I do not know how safe this is. Good luck
Thank you Fabio Fornari Md. Atikul Islam Timothy Omara for sharing your answers.
Md. Atikul Islam Our goal is to do phytochemical screening of few specific plant leaves, both qualitative and quantitative, and to choose the ideal MS for such purpose.
Dear Arpita dey
Thank you for shearing your objective
1. For phytochemical screening ---first you may try chemical test ( Qualitative analysis) such as example phenol, flavonoid, terpene, phytosterol, and saponins, etc.
secondly----You quantify their total phenol, total flavonoid, antioxidant activities (quantitative analysis)
thirdly---You may used HPLC-DAD or LC-MS/MS for your target compound analysis.
2. GC-MS sample preparation and analysis is different objective.......
For sample preparation .....you may use, Super critical fluid extraction, Hydro distillation, Stem distillation, Simultaneous distillation extraction with solvent, Solid phase extraction , etc.............It was depend on your facility and target what you want ??
You need more information ...please share your theme
Than you
I agree with the solution provided by Md. Atikul Islam, GC-MS even though you can certainly utilize (Article GC–MS analysis of phytoconstituents from Amomum nilgiricum a...
), may not be an ideal analytical technique for your described goal - qualitative and quantitative phytochemical analysis. Rather as suggested, HPLC / LC-MS analysis following biochemical qualitative & quantitative analysis may provide better results (Article Phytochemicals: Extraction, Isolation, and Identification of...
)
Khalid Muzaffar, Thank you for your suggestion and the reference articles.
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