Salkowski reagent preparation?

More Rihab Djebaili's questions See All

Generated the wave function file (.wfn) using Gaussian 09W ?

Dear all, I would like to generate a wave function file (.wfn) using Gaussian 09W. I have added output=wfn in route section, and at the end of the molecular coordinate section i leaved blank...

17 February 2020 3,621 4 View

Analytical techniques for freeze drying process monitoring?

Hello, Many techniques are reported in the literature for monitoring various parameters of a freeze drying process. For example, I have come across: - Freeze drying microscopy - Tunable Diode...

21 December 2018 4,155 0 View

EFA in R studio?

Hi, I need to do exploratory factor analysis in R studio. The Cronbach's alpha is low (0.5). There is no way of bringing it up, even by removing questions with poor corelations. I used a...

20 May 2018 5,457 5 View

Any advice to align two hierarchical structure of fuzzy clusters?

About aligning and merging two hierarchical structures of fuzzy clusters, i'm looking at previous works which have treated like this problem? any help

10 August 2015 1,064 2 View

What is the effect of a low 260/230 ratio on the purity of DNA?

I have a number of samples of DNA extracted from blood on a filter paper with low 260/230. Does this low ratio inhibit the amplification of the DNA to form a band on the agarose gel after a PCR run?

04 March 2014 3,528 23 View

What are the advantages of using a ROC curve?

I am comparing three diagnostic tests in human. How could a ROC curve help me to determine which is the best and how I could make use of all the data I get when I use SPSS?

20 February 2014 9,324 6 View

I requested a paper from an author via e-mail, but no response. Any clues as to why?

I was interested in a paper and found the author's email in a different paper, and sent a request to the author, but have not had a reply. A colleague of mine had the same experience. Does anyone...

05 January 2014 4,599 19 View

What lysis buffer to use to extract DNA from filter paper using qiagen kit?

I want to extract DNA from blood found in whatmann number 3 filter paper using qiagen kit. The kit lacks the buffer that lysates the blood prior to extraction. Is there a lysis buffer I could use...

30 December 2013 8,250 8 View

Does anyone work on Sobol sensitivity analysis?

I tried to apply Sobol 2002 and 2007 sensitivity analysis to a LSM to evaluate for the different parameters the first order and total sobol index respectively Si and ST. But I always find non...

13 June 2013 9,151 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

How to select target material for X-Ray generation?

Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?

31 July 2024 5,824 0 View

Metwally Ramadan Kottb

Perchloric acid 35% is better than sulfuric acid

Wendesen Melak Tesema

Salkowski's reagent originally prepared with sulphuric acid has a short life span. It needs to be read the sooner the pink color forms when read in a spectrophotometer(530nm), but when perchloric acid is used, it can stay stable before the color fades, for a few hours with stability.