Please for preparation of Salkowski reagent to estimate IAA production on liquid medium, is better to prepare it with perchloric acid and iron chloride, or with sulfuric acid and iron chloride?
Salkowski's reagent originally prepared with sulphuric acid has a short life span. It needs to be read the sooner the pink color forms when read in a spectrophotometer(530nm), but when perchloric acid is used, it can stay stable before the color fades, for a few hours with stability.