Hello,

I have been trying to write a shell code for a 1-way ANOVA analyzing gene expression levels over multiple days. The data we will be running will most likely be with biological replicates, so I do not want to average the RPKM values together across replicates. This is an example of what the table would look like:

day 1 day 2 day 3

Gene 1,2,3 1,2,3 1,2,3

A

B

C

So 3 biological replicates over 3 days. I do not want to compare across genes but rather compare within gene for expression levels across days. I appreciate your help in advanced