Good day everyone,
I had a problem with my RT PCR machine, while it was performing it stopped suddenly because of another program, and I didn't get a chance to resume it (Although there is no option like this), and it reached cycle number 18 out of 40 cycles. while I was searching in the settings I found an option called quick start and I started my run over again. In this case, should I expect any right results?
Hi Sebastian Schmitt
Thank you for the reply,
I think I will repeat it because there is no amplification curve at all compared to the 18 cycles of the real run.
Have a nice day,
Khawla
repeat the reaction and compare it with the last one I am sure this will be added for your experience.
Dear Manal Al-nasrawi
thank you for your reply,
I repeated the reaction and I knew what should i get but the amplification curve showed wrong results after the machine stopped.
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