Khawla Saif

11 Questions 17 Answers 0 Followers

Questions related from Khawla Saif

How to set a proper method of images thresholding using Fiji ImageJ?

Hi everyone, I am using Fiji in order to get IntDen. of some cancer tissues, and I’m following these steps, drag the image Edit, selection, Specify, W=250 H=250. image, color, colour...

30 April 2023 2,639 3 View

I found my protein bands are in higher sizes than expected in western blotting, do you know the reasons?

21 April 2023 4,918 3 View

Cell Viability Assay

hello everyone, I will be conducting an RNU assay, some papers mentioned that they prepare the RNU mixture one day before and incubate it at 37C overnight, but the protocol directs me to prepare...

01 January 1970 6,488 0 View

RT PCR system

Good day everyone, I had a problem with my RT PCR machine, while it was performing it stopped suddenly because of another program, and I didn't get a chance to resume it (Although there is no...

01 January 1970 9,708 3 View

Western blot Bands

Hi all, I did a western blot and I got these bands of GAPDH although my target protein bands are good (SOCS6 and EpoR) any explanation?

01 January 1970 2,432 8 View

Protien Quantification for western blot

Hello everyone, I did BCA for SOCS6 protein for the first time, some samples showed a concentration higher than the highest standard. what should I do in this case, should I go for a nano-drop to...

01 January 1970 4,868 5 View

Western blots preservation

Hi all, I wanted to ask about the best method of blots long-term preservation so it can be handed to the reviewers later, and if it is better to be stripped or not! Thanks in advance!

01 January 1970 392 4 View

HEK 293 FT cell maintaing

Dear All, Good day I trypsinized HEK 293 FT today and split them into two flasks but after loading the needed amount we noticed that cells are not separated well and some are in clumps, would...

01 January 1970 6,103 4 View

WB loaded protein concentration

Hi all, I loaded 40 ug of HT-29 cell line protein, and I couldn't find my target band. But when I incubated it with the housekeeping gene, while detecting it showed two bands- faint bands for...

01 January 1970 1,490 3 View

Skin fibroblast and stem cells

Hi all, Good day, I am new to Skin fibroblast and stem cells, what type of media is preferred to be used, as per my reading I found some are using DMEM, and others are using Fibroblast Growth...

01 January 1970 4,291 0 View

Differentiation of iPSC into cardiomyocytes

Hi all, I will soon start dealing with iPSCs and try to differentiate them into cardiomyocytes. Any tips I should put in mind while working? Or good papers to refer to? Thanks in advance,

01 January 1970 9,862 0 View