How can I detect IL-1B by Western blot?

Hello Everyone, I know IL-1B is a soluble protein. So how to extract it from the supernatant? Please advice. Thanks!

05 January 2021 7,349 5 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Are there any EEG facilities for hire?

I need to run a quick EEG experiment. I don't want to spend time and money to set my own lab. I wonder if there exist EEG labs that can run an experiment for me for a fee?

10 September 2020 5,492 3 View

How do you report undetermined Ct values?

I have knocked down a gene of interest using siRNA and am using qRT-PCR to confirm reduced expression. All samples treated with non-specific siRNA have Ct values around 35, but all samples...

29 July 2020 8,198 3 View

Use of spherical silver?

Where is silver in spherical shape used other than for solar cell electrodes? Most of the silver in electronics seems to be flake. Is this statement accurate?

24 May 2020 7,604 3 View

Insect Population Counting and Studying Traps - recommend a good book or source for info on?

Hi, I'm looking for an entomologist that can recommend a good book on the trapping of insects for study. Thanks

30 January 2020 2,710 2 View

IHC: Monocyte vs Macrophage?

How would you distinguish peripheral monocytes from tissue macrophages with IHC? Specifically, I am interested in looking at rodent brain tissue. Any help is appreciated! Thanks, Dan

07 October 2019 2,683 3 View

Where can I find data on urban land cover in the UK from the 1960s?

I'm modelling insect populations over time (1968 - present) in the UK at over 500 sites. I have detected a decline in sites that are mainly suburban (based on 500m radii using the CEH Land Cover...

02 September 2019 535 3 View

Would (groundwater) dissolved noble gas concentration change after isolation from gas exchange with air?

Dear scientists of noble gas and hydrology, Would equilibrium and/or excess concentrations of noble gases change after isolation from gas exchange with atmosphere? (Non-atmospheric noble gas was...

20 February 2019 255 5 View

Is there a way to control for categorical groups to calculate partial correlations for various measurements?

I have a sample size of 30, representing three different groups (one intervention and two controls). I would like to see if there is an association between my dependent variable and 4 self-report...

18 February 2019 4,619 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View