Rose bengal medium ?

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Enzyme activity not working?

I am trying to do an enzyme assay using Wild type enzyme the protocol involves tris buffer pH 8, MgCl2, S-adenosyl methionine, Substrate, and I inititate the reaction with my enzyme, but after...

06 July 2019 6,878 3 View

Protein size decreases after mutation?

I am trying to express mutants of my protein whose size is 30 Kda... however following purification by Ni affinity column ... the SDS PAGE shows the mutants appearnig at size 20 Kda.. is there a...

04 May 2019 5,066 15 View

BL21 pLyss transformation and chloramphenicol?

Do I need to add chloramphenicol to LB agar media when trying to transform my plasmid into BL21 pLyss or I can add only the antibiotic of my plasmid?

04 May 2019 9,238 5 View

Why protien not eluted after changing buffer ?

II am currently working on a protien to be crystallized. The protien is bound to FAD, that's why when I purify the protein with Ni affinity column its colour was yellow . but surprisingly, after...

07 August 2018 4,340 4 View

How to make protien soluble during expression ?

I am trying to express and purify a protein after doing all the steps and purify using Ni affinity column but after doing the SDS PAGE I found that the amount of protein in the elute is very...

05 June 2018 8,574 6 View

Use of precukture ?

I want to ask why we always do a starter preculture step during protein expression ? Why don't we just start the main culture directly ?

04 May 2018 4,572 2 View

Protien not purified using ion exhange?

I am trying to purify a protein..First I run Ni affinity column and the protein was eluted with KPB pH7.9, imidazole 500mM and glycerol ..then I diluted with No Salt buffer containg 50mM TrisHCL...

04 May 2018 5,996 8 View

Chemical shift of acetyl gp?

what is the carbon nmr chemical shift of methyl gp in acetyl gp

11 December 2017 9,505 3 View

Methoxy gp on aromatic ring ?

If i have an aromatic ring with methoxy gp on it and 3 aromatic protons in ortho, meta and para position ..Is there an explanation for why the ortho proton appears at lower chemical shift than...

09 October 2017 8,881 3 View

Isolation of soil microbes?

I have cultured soil on 2 medium .. humic acid medium and chitin agar medium for culture of fungi and actinomycetes.. the question is .. how can i know the colon grown is fungi or bacteria .....

08 September 2017 4,154 6 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View