Is anyone familiar with denaturation of proteins in native gel?

I have an antibody recognizing my protein in its unfolded state, so I can visualize it by western-blot after SDS-PAGE. Unfortunatly, I can't see it after native-PAGE to follow its...

08 September 2014 492 7 View

How can I avoid HEK cells from forming clumps?

I generated stable HEK lines, but some lines tend to clump (others not), and when I try to separate the cells by pipetting, lots of them dye. As I intend to run flow cytometry, I guess cell...

03 April 2013 7,989 5 View

Are HEK293 Free Style cells suitable for stable selection?

I want to use HEK cell for stable selection, I know 293T are not suitable, and I found a batch of 293F (supposed to be cultured in suspension but that seem to grow very well as adherent cells...)....

01 February 2013 4,888 2 View

HEK293 or 293T for stable line selections with Geneticin?

I want to select stable HEK with Geneticin in order to express a membrane receptor and run functional studies (overexpression is not important). I read that HEK293Ts are not the best cells for...

31 December 2012 2,743 12 View

What is the risk, in terms of stability, to select stable HEK293 lines with a plasmid containing SV40 origin?

My plasmid contains a SV40 origin and a Neo selection marker; if I select 293T cells I have to use another antibiotic (and make a co-transfection with a second plasmid for the selection), if I use...

31 December 2012 2,043 1 View

How long can you culture HEK293 cells for?

They are not immortalised, contrary to 293T cells.

31 December 2012 5,607 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

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02 March 2021 3,060 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View