Philippe Leone

7 Questions 3 Answers 0 Followers

Questions related from Philippe Leone

RNAse-free filter tips: use despite expiration date that has run out?

I have a lot of RNAse-free filter tips with an expiration date that has run out since a more or less long time (2017 and even 2014!). Do you think I could I use them anyway without much risk to...

12 December 2018 7,311 0 View

Is anyone familiar with denaturation of proteins in native gel?

I have an antibody recognizing my protein in its unfolded state, so I can visualize it by western-blot after SDS-PAGE. Unfortunatly, I can't see it after native-PAGE to follow its...

09 September 2014 545 7 View

How can I avoid HEK cells from forming clumps?

I generated stable HEK lines, but some lines tend to clump (others not), and when I try to separate the cells by pipetting, lots of them dye. As I intend to run flow cytometry, I guess cell...

04 April 2013 8,028 5 View

Are HEK293 Free Style cells suitable for stable selection?

I want to use HEK cell for stable selection, I know 293T are not suitable, and I found a batch of 293F (supposed to be cultured in suspension but that seem to grow very well as adherent cells...)....

02 February 2013 4,938 2 View

What is the risk, in terms of stability, to select stable HEK293 lines with a plasmid containing SV40 origin?

My plasmid contains a SV40 origin and a Neo selection marker; if I select 293T cells I have to use another antibiotic (and make a co-transfection with a second plasmid for the selection), if I use...

01 January 2013 2,083 1 View

How long can you culture HEK293 cells for?

They are not immortalised, contrary to 293T cells.

01 January 2013 5,655 2 View

HEK293 or 293T for stable line selections with Geneticin?

I want to select stable HEK with Geneticin in order to express a membrane receptor and run functional studies (overexpression is not important). I read that HEK293Ts are not the best cells for...

01 January 2013 2,819 12 View