Sometimes two plant genes (A and B) contain high Homology[95~98%],whether can use RNAi to Downregulate A gene at the same time but not affect on B.How to select the RNAi region?
Sometimes the whole gene region contains high GC content[~80%],it is difficult for Real Time PCR to analyze the simples;I have tried to design several primers to optimizate the results,but they...
11 December 2012 8,039 6 View
Sometimes two genes contain high Homology[95~98%],it is difficult for Real Time PCR (SYBR Green method)to design primers,and it seems not possibile to distinguish the Major gene .Can somebody...
11 December 2012 9,485 4 View
I want to know whether by the way of Real Time PCR can distinguish the target gene's expression level Between the RNAi Transgenic lines and WT, if can do, how to do?
11 December 2012 5,260 2 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View